SURVEYOR^® Mutation Detection Kit for Universal Primer Fluorescent Capillary Electrophoresis

Finding the Needle Just Got Easier

SURVEYOR Mutation Detection Kit for Universal Primer Fluorescent Capillary Electrophoresis detects mutations in DNA amplified with two fluorescent primers when digested with SURVEYOR Nuclease for subsequent analysis by fluorescent capillary Electrophoresis using an ABI PRISM^® Genetic Analyzer and ABI GeneScan™ software.

Benefits

• Determines mutation loci
• Saves time and money
  – Streamlines DNA sequencing
  – Decreases sequencing backlog and analysis time
  – Validates sequencing results and analysis
  – Decreases chromatogram analysis by 90%
• No post-PCR or post-reaction clean-up
• High through-put sensitivity and specificity

Designed for Multiple Mutation Discovery Applications

• Somatic, germ-line and mitochondrial mutations
• Insertions, deletions, substitutions and SNPs
• Low-copy mutations and pooled DNA samples
• Taq polymerases introduce errors (i.e. mutations) into the amplicon that contribute to background. High fidelity proofreading polymerase blends are recommended. Use Maximase Polymerase for high fidelity PCR.

Products

SURVEYOR Mutation Detection Kit for Universal Primer Fluorescent Capillary Electrophoresis
                            Quantity                         Catalog No.
                        32 reactions                         706101
                        96 reactions                         706102
                        384 reactions                       706103

SURVEYOR Mutation Detection Kit for Universal Primer Fluorescent Capillary Electrophoresis with Maximase™ Polymerase and dNTPs
                            Quantity                         Catalog No.
                        32 reactions                         SP2070
                        96 reactions                         SP2071
                        384 reactions                       SP2072

Maximase™ Polymerase (250 units) for 100 reactions Catalog No. 703245