Navigator Software brings together the control of the WAVE
System with MutationDiscovery.com™, a resource for scientists
doing research in the area of genetic variation. The site
includes entries for thousands of genes, and each entry presents
known variations in the context of the genomic sequence for
the gene. Navigator Software uses a sophisticated relational
database management system to store and run internal procedures
to collect results. This along with the object-oriented software
design, provides the ability to easily develop additional
instrument interfaces and software modules.
Powerful proprietary algorithms are used
for the prediction of optimum denaturation conditions
for mutation detection
based on DNA fragment length, nucleotide sequence and
composition, and chromatography conditions. These features
provide the
user with superior data analysis and reporting capabilities.
Navigator Software works in conjunction with the WAVE System
to provide outstanding mutation detection capabilities while
ensuring data integrity. Multiple chromatograms from a project
can be overlaid with a wild type providing rapid comparison
of a large number of samples. Chromatograms can be exported
for use with programs such as Microsoft Word®, Excel® and
PowerPoint® for manuscript preparation or presentations.
MutationDiscovery.com
Transgenomic's web site, www.mutationdiscovery.com,
provides subscribers with seamless access to experimental
methods for use in Navigator Software. Researchers
may view
methods and validation data for detecting peer reviewed
(published) variations in specific genes. Sequences and amplicons
may be imported from mutationdiscovery.com into Navigator
Software. Variants and features may be identified and analysis
conditions determined.
WAVE® System Data Analysis
Navigator Software includes advanced data analysis capabilities.
Data may be retrieved from multiple projects and trays and
then compared manually against a known wild type or analyzed
using powerful mathematical algorithms. The traces can then
be grouped and reports produced.
One of the most useful and timesaving features of Navigator
Software is its computer-assisted mutation-calling program.
Navigator Software allows you to compare hundreds of samples
against each other; the sample traces are first normalized
and then mathematically discriminated into clusters using
Principle Component Analysis. This program separates wild
type and mutant traces with amazing accuracy, eliminating
hours of post-analysis data reviewing. Comprehensive reports
can be generated, showing both the grouping of samples in
a table and also in a three dimensional scattergraph.
Network Solutions
With remote access you can access the database of any WAVE
System in the connected network location.
This allows
you to
set-up projects, run them, analyze them, and report your
findings all from a remote location which may include your
office. (Lab personnel must be available to physically place
the selected
tray(s)
in
the autosampler.)
Backward Compatibility
You may view Wavemaker Software data with the included
Wavemaker Software desktop version. That data may also be
imported into Navigator Software projects.
Features
NEW for Navigator
Software version 1.7.0
Protocols and Protocol
Templates allow complete automation and
pre-programming of all assay
parameters including amplicons and methods,
physical tray configurations and analysis
parameters used in mutation calling.
Data can be exported to a pipe-delimited
text file allowing easy integration with third
party tools.
Progress reports summarize real-time
completion statistics. Ideal for multi-amplicon
assays.
The 3D scattergraph and affinity
tree with cluster analysis can be saved in PDF
format or printed in a mutation calling report.
Generates context specific reports of sample
analysis which can be saved in PDF format or printed.
Select
results for analysis from any project or tray in
the Navigator Software database.
Automatically predicts analysis conditions,
such as temperature and buffer gradient based on
analysis mode.
Detects the presence or absence of mutations
using discriminate analysis.
Mutation
calling enables you to sort data into groups
of wild-type or mutants.
View real-time results.
Sequences and amplicons can be imported from
MutationDiscovery.com.