Optimase^® Polymerase

For high-fidelity PCR in mutation detection, SNP discovery, and high-fidelity cloning

Optimase Polymerase is a thermostable DNA polymerase with an efficient 3' to 5' proof-reading exonuclease activity developed for unprecedented PCR performance in single nucleotide polymorphism (SNP) and mutation discovery studies. Initially developed for denaturing high performance liquid chromatography (DHPLC) research^1-4, Optimase Polymerase is the enzyme of choice for other applications requiring high fidelity PCR amplification.

View FAQs about Optimase Polymerase

Applications

• SNP and mutation discovery and scoring with various techniques such as SURVEYOR^® Nuclease genome scanning, DHPLC, SSCP, DGGE and others
• High fidelity cloning

References

1. Muhr, D., Wagner, T. and Oefner, P. 2002. Polymerase chain reaction fidelity and denaturing high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci, 782:105-110. Abstract Library Entry
2. Nickerson, M. 2003. “Influence of PCR on DHPLC Polymorphism Characterization.” In Hecker, K. (Editor): Genetic Variance Detection: The Nuts&Bolts of DHPLC in Genomics.. 2003, DNA Press.
3. Application Note #119u. 2002. Transgenomic Optimase Polymerase delivers highest fidelity in PCR for WAVE^® System analysis. http://www.transgenomic.com
4. Jagmohan-Changur, S., et al. 2003. EXO1 variants occur commonly in normal population: evidence against a role in hereditary nonpolyposis colorectal cancer. Cancer Res, 63:154-158. Abstract Library Entry
5. Mo, J.Y., Maki, H., and Sekiguchi, M. 1991. Mutation specificity of the dnaE173 mutator associated with a defect in the catalytic subunit of DNA polymerase III of Escherichia coli. J. Mol. Biol. 222: 925-936.