Analysis Conditions

• Buffer A: 0.1% Trifluoroacetic Acid in H₂O
• Buffer B: 0.1% Trifluoroacetic Acid in Acetonitrile
• Gradient: 20% - 50% B in 15 minutes
• Flow Rate: 0.5 ml/min
• Temperature: 80°C
• Protein Concentration: 0.4 mg/ml;C
• Injection Volume: 20 µL

Sample Points

1. Ribonuclease A
2. Cytochrome C
3. Lysozyme
4. Myoglobin
5. Chymotrypsinogen A