The WAVE System Model 4500HT-HS is designed with higher through-put labs
in mind and labs requiring the most sensitive analysis
possible for low-level mutation detection. The WAVE System is
one of the most sensitive and accurate technologies for sequence
variant analysis and separation of nucleic acid fragments. The
WAVE 4500HT-HS System is fully automated, from sample collection
to results analysis, and is able to analyze samples twice as
fast as the standard WAVE 4500 System. Navigator™ Software
creates the fragment specific methods, controls the instrument
and analyzes your results. Fragment separation is achieved by
the patented DNASep® HT Cartridge technology.
The fragments are intercalated with fluorescence dye and pass
through the system’s fluorescence detector. The fluorescence
level is recorded over time, and this information is processed
by the Navigator Software suite. It records the results in the
form of chromatograms that can be further analyzed and filed by
Navigator.
WAVE HSD - High Sensitivity Detection Module
Any WAVE 4500 System may be upgraded to a WAVE HS System by adding the WAVE HSD. The WAVE HSD consists of:
- WAVE FDX - Fluorescence Detector (see description above)
- WAVE HSX - High Sensitivity Accessory
The WAVE HSD incorporates fluorescence detection and intercalation chemistries to provide sensitivity
enhancements for the detection of ds- and ss- DNA and RNA without fluorescently labeled primers. The HSD significantly
increases signal sensitivity, typically by orders of magnitude when compared with UV detection.
The WAVE HS System can be used for applications that require extra sensitivity, including the following:
- Detection of somatic mutations - enhances the detection of mutant alleles at low concentrations
in the presence of highly abundant wild type alleles, in excess of 1:100 ratio
- Pooling of DNA samples - detects mutations in plant samples (barley) at a 1:48 ratio of mutant to
wild type plants
- Analysis for samples originiating from PCR with low template concentration - PCR samples containing DNA
template with concentrations as low as 0.5 ng can be successfully analyzed.
This accessory module may be added to a current WAVE 4500 System or a system upgraded to high sensitivity
at the time of purchase.
WAVE 4500 System Model Comparison -
Click here
