Solid Phase Extraction

Transgenomic POLYSorb™ Products for Solid Phase Extraction

Solid Phase Extraction (SPE) is a sample preparation technique that is employed to clean up or concentrate samples prior to analysis. SPE can be used to clean-up samples by removing interferences that would otherwise compromise analysis. It can be used to concentrate by allowing a large volume of sample to be reduced into a small elution volume. Compared to other sample preparation techniques, such as liquid-liquid extraction, SPE provides cleaner extracts with high recoveries. SPE is also faster and uses less solvent which saves money.

Modes

SPE tubes can be used in two modes:

1. In the flow-through mode the sample can be passed through the tube. While passing through the tube, the contaminants present are retained while the analyte of interest is allowed to pass through. The steps for this mode are 1) Load the sample into the tube 2) Wash to elute the analyte of interest.
2. In the selective elution mode the sample is passed through the tube. But in this mode, the analyte of interest is retained while contaminants pass through. After the sample is loaded onto the column, the analyte of interest is selectively eluted by choosing elution conditions that will elute the analyte from the column while retaining interfering components.The steps used with this mode are 1) Load the sample onto the column 2) Wash through weakly retained or unretained contaminants 3) Elute the analyte of interest.

The most common SPE packing are polar adsorbants. These adsorbants are used to remove organic interferences from samples. Also, commonly used are ion exchangers to remove charged species as interferences. Transgenomic offers products for both adsorption and ion exchange.

Key Features of Transgenomic SPE products

As with all of Transgenomic’s chromatography products, the SPE products are all based on polymeric resins. Polymer-based resins are used because of the broad pH range available and the chemical and physical stability of the materials. These cartridges are ideally suited for cleaning up samples in tough matrices.

Transgenomic POLYSorb cartridges provide very high loading capacities to accommodate for concentrated samples. POLYSorb cartridges also provide excellent selectivity even for trace level analysis.

POLYSorb Cartridges in the format you need

Transgenomic POLYSorb cartridges are provided in three stationary phase formats:

• Unmodified Poly-[styrene/divinylbenze] (PS/DVB)
• Alkylated (C18) PS/DVB
• Sulfonated PS/DVB

Transgenomic offers each of these cartridges in either 100mg or 400mg tubes, or we can custom pack in sizes to meet your specific needs.

POLYSorb tubes are compatible with off-the-shelf SPE vacuum manifolds, automated workstations or other commonly used accessories.

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