Polymeric Reversed Phase
RPSep Columns
Reversed phase is commonly referred to as adsorption
chromatography. Reversed phase works by taking advantage of
the hydrophobic interactions between molecules and a
hydrophobic stationary phase.
In reversed phase, molecules are adsorbed onto a hydrophobic
stationary phase.Then, the molecules are desorbed by changing
the hydrophobic character of the mobile phase such that the
molecules will selectively partition into the mobile phase and
elute from the column.
Traditionally, silica-based packings have been the most commonly
used sorbants. However, as samples become more challenging, as
with biological samples, supports are required that have broader
pH ranges, are more rugged, and can be cleaned.Transgenomic
provides a family of products all based on polystyrenedivinylbenzene
sorbants that utilize our patented alkylation technology.
Features
The key features of RPSep polymeric reversed phase columns are:
- pH stable from 0 – 14
- temperature stable
- very rugged, long lasting materials
- very tight particle size range (± 0.5µm) for high efficiency
- very high efficiency for polymeric resins
- both alkylated and non alkylated PS/DVB available
- all resins available in both analytical and bulk for scalability
And, as with all Transgenomic Chromatography products, RPSep
columns provide excellent column-to-column and
lot-to-lot reproducibility.
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