Amino Acid Analysis
Transgenomic Columns for Amino Acid Analysis
Ion-exchange chromatography is a popular technique for the
analysis of amino acids because both retention times and
quantification are highly reproducible regardless of the sample
matrix. This unique matrix insensitivity is important when
comparing results from different patients or batches of protein hydrolysate.
Amino acids are zwitterions; at low pH, they are positively-charged
and are bound to the resin by their attraction to the negatively-charged
ion-exchange sites. Almost all the contaminants, i.e.
matrix, are eluted at the void.The amino acids are then selectively
eluted by increasing the pH and salt concentration with different
buffers.With few exceptions, the order of elution follows the
isoelectric point of the amino acids, i.e. acidic amino acids first,
then neutral and basic. Because the separation and the ensuing
post-column reaction of amino acids are devoid of contaminants,
amino acid analyses via ion-exchange chromatography are highly reproducible.
Features
The key features of the Transgenomic cation-exchange columns are:
- Polymeric Substrate
- High efficiency
- High resolution
- Reproducibility lot-to-lot and column-to-column
- Rugged
- Available for both physiological and protein hydrolysate amino acids
Amino acid columns are subjected to many different types of
samples (blood, urine, growth media, animal feed, wine, etc.) and
often they are introduced with minimum sample preparation.
Therefore this variety of matrix challenges all but the most rugged
ion-exchange columns.Transgenomic columns use
polystyrene/divinylbenzene copolymers and are stable in the pH range
of 0 to 14; they are temperature stable and very rugged.
The Transgenomic amino acid columns have been shown to last
for thousands of runs without cleaning. Because Transgenomic
manufacture the polymers and pack the columns, lot-to-lot and
column-to-column reproducibility is excellent (retention times
vary by less than 1%). Available for both routine hydrolysate
analysis as well as complex physiological fluids, Transgenomic
amino acid columns have been designed to provide the highest
efficiency and highest resolution of any ion-exchange amino acid
columns on the market.
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