Microbial AnalysisOverviewTraditional microbiology techniques rely primarily on agar culturing as a means to enumerate and identify bacteria, especially when analyzing complex, mixed species cultures. In addition to being time consuming (12-72 hours), many bacteria, such as fastidious and anaerobic bacteria require specific growth conditions and it is estimated that in some samples (e.g. environmental samples) less than 10% of bacteria are successfully cultured. Due to the constraints of traditional techniques, Microbiologists have recently turned to genetic analysis techniques to help profile and identify the bacterial and fungal diversity in a variety of different clinical and environmental samples. In addition molecular techniques have been used to characterize antibiotic resistance via Mutation Detection analysis. The WAVE® Microbial Analysis System offers full automation, high throughput and yield high reproducibility and can be used to solve these microbiological questions. The WAVE System enables separation of PCR products of similar or identical size but of different sequence using Temperature Modulated Fragment Analysis (TMFA). To date the most widely used mode of TMFA is DHPLC. The WAVE System with its Navigator™ Software also allows automated fragment collection of the separated PCR products for further molecular analysis such as DNA sequencing, cloning, etc. Navigator™ Software maintains an electronic record of the fragment collection parameters such as fragment destination (vial), volume collected and collection parameters. (Fragment Collector) ApplicationsWAVE Microbiology Applications Publications |