SURVEYOR
™
Nuclease Digestion of
Heteroduplexes with Different Insertions
_______ Insertions in Test Amplicons_________
1 bp
2 bp
3 bp
6 bp
9 bp
12 bp
Ctrl
M
S
P1
P2
S
= 632 bp Heteroduplex and Homoduplex Substrate
P1 = 415 bp Cleavage Product
P2 = 217 bp Cleavage Product