CRC Tumor APC 1430
• Genomic DNAs from control and
colorectal tumor cell-lines were mixed at various ratios
from 1:1 to 1:499 (wild-type:mutant)
• A 300 bp region from the APC mutation
cluster region (MCR) of exon 15 was amplified by PCR
using Optimase® Polymerase
• PCR products were analyzed for
mutations using SURVEYOR Nuclease methodology
• SURVEYOR Nuclease cleavage products
were detected using the WAVE-HS platform
Detection of Variants in the APC Mutation Cluster Region Using SURVEYOR™ Nuclease
