DHPLC-based Methylation AnalysisDNA methylation contributes to the epigenetic control of gene activity in many organisms. These heritable variations in gene function are not caused by DNA sequence alterations but rather rely on chromatin modulation. Such control is of significance for a broad scope of biological processes, ranging from cellular differentiation during development and the establishment of monoallelic gene expression in genomic imprinting and X-chromosome inactivation to the suppression of parasitic sequences and the maintenance of genome stability. WAVE® System approaches: - Reveal differences in DNA methylation by monitoring retention times of amplicons derived from bisulfite treated DNA under partially denaturing (DHPLC) conditions.
- SIRPH (SNUPE, single nucleotide primer extension, combined with IP-RP-DHPLC) monitors quantitative changes of methylation at specific CpG sites of interest. It relies on single nucleotide primer extension performed with bisulfite treated, PCR-amplified DNA.
|