Mutation Detection and SNPs Discovery with DHPLCThe Transgenomic WAVE® System Instruments utilize DHPLC (denaturing high-performance liquid chromatography), which is among the most sensitive and accurate technologies for detection of unknown genetic mutations and single nucleotide polymorphisms (SNPs). DHPLC is based on DNA heteroduplex formation and separation of heteroduplex from homoduplex molecular species by means of ion-pair reverse phase HPLC carried out with the Transgenomic patented separation DNASep® cartridges. A series of WAVE system instruments meet the throughput requirements of all levels of genetic variation screening projects. The WAVE System is controlled by the proprietary Navigator™ Software, which predicts analytical parameters with a high accuracy for optimal fragment separation and mutational analysis. Data and protocols can be uploaded and shared with the scientific community on the www.MutationDiscovery.com web-available database. Benefits of using DHPLC- Discover unknown genetic variants without sequencing at a lower cost
- Sequencing can not find all mutations in heterogeneous samples due to the low abundance of variant alleles – DHPLC can
- Robust technology that is used in clinical diagnostics environment
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