Sample Preparation

Dilute wine 1:10 with distilled water

Conditioning Step

Wet tube with 1 mL of methonal followed by 1 mL of 10:90 methonal:water

Sample Addition

Load 500 µL of dilute wine

Wash Step

1.0 mL of water

Elution Step

1.0 mL of 0.05 N H₂SO₄

Analysis Conditions

• Column: ICSep ION-300
• Eluent: 0.01 N H₂SO₄
• Flow Rate: 0.5 mL/min
• Temperature: 60°C
• Detection: UV at 214 nm
• Injection: 20 µL

Sample Points

1. Citric Acid
2. Tartaric Acid
3. Glucose
4. Malic Acid
5. Fructose
6. Glycerol
7. Succinic Acid
8. Acetic Acid