Analysis Conditions

• Column: RPSep Poly-RP C0
• Eluent: 0.1 M Ammonia in 80:20 ACN:H₂O
• Flow Rate: 0.75 mL/min
• Temperature: Ambient
• Detection: UV at 210 nm

Sample Points

0.05 µL/mL of

1. Trimethylamine
2. Triethylamine
3. Diisopropylethy-lamine
4. Tripropylamine
5. Tribitylamine