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Separation of polar and Non-polar Compounds

Chromatogram

Analysis Conditions
  • Column: RPSep ACT-1 C18
  • Eluent: 60:40 ACN:H2O
  • Flow Rate: 0.3 mL/min
  • Temperature: Ambient
  • Detection: UV at 254 nm
 
Sample Points
  1. Unknown
  2. Phenol
  3. Aniline
  4. Acetophenone
  5. Nitrobenzene
  6. Toluene
 
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