Analysis Conditions

• Column: RPSep Poly-RP C0
• Eluent: 0.01 M KH₂PO₄ in 25:75 ACN:H₂O
• Flow Rate: 0.75 mL/min
• Detection: UV at 254 nm
• Injection: 10 µL

Sample Points

1. Sulfanilic Acid (10 µg/mL)
2. Sulfanilamide (10 µg/mL)
3. Sulfathiazole (20 µg/mL)
4. Sulfamethizole (20 µg/mL)
5. Sulfamerizine (30 µg/mL)
6. Sulfamethazine (30 µg/mL)
7. Sulfisoxazole (30 µg/mL)
8. Sulfamethoxazole (30 µg/mL)