PCR for Mutation Detection

DHPLC has emerged as the most sensitive physical mutation screening method available.¹ Some early studies with DHPLC reported a small number of false positives. These false positive calls were related to poor PCR quality. Proper use of a proofreading DNA polymerase such as Optimase eliminates such issues.²

Enzymatic mutation scanning with SURVEYOR Nuclease has also shown that use of high-quality amplified DNA with a minimum of PCR errors is essential to robust, reliable mutation calling.

Generally, the quality of amplified fragments exceeds that required for sequencing or subcloning. Transgenomic developed high fidelity Optimase Polymerase to meet this high quality standard.

Transgenomic's Optimase Polymerase and its buffer systems have been designed to ensure

the longest DNASep cartridge lifetime and
the highest possible reliability of mutation calling through high-fidelity DNA amplification.

References

Eng C, Brody LC, Wagner TM, Devilee P, Vijg J, Szabo C, Tavtigian SV, Nathanson KL, Ostrander E, Frank TS (2001) J. Med. Genet. 38:824. Abstract Library Entry
Muhr D, Wagner T, Oefner PJ (2002) J. Chrom. B 782:105. Abstract Library Entry

These and other publications can be found in the Transgenomic Journal Articles library.