Analysis Conditions

• Column: ICSep ORH-801 packing L17 specification
• Eluent: 0.01 N H₂SO₄
• Flow Rate: 0.6 mL/min
• Temperature: 37°C
• Detection: UV at 210 nm
• Injection: 20 µL

Sample Points

USP Malic Acid (100 mg in 100 mL volumetric flask, made up with 0.01 N H₂SO₄)

1. Maleic Acid
2. Malic Acid
3. Fumaric Acid